Occupational Safety and Health Administration (OSHA) SLC Practice Exam

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What is an autoclave used for?

  1. To clean surfaces with disinfectants

  2. To store medical instruments

  3. To kill microorganisms using steam

  4. To transport biological samples

The correct answer is: To kill microorganisms using steam

An autoclave is specifically designed to kill microorganisms through the use of high-pressure steam and elevated temperatures. This process is essential in settings such as medical and laboratory environments where sterilization of equipment and materials is vital to prevent infection and contamination. The steam penetrates the items being sterilized, raising their temperature to a level that effectively destroys bacteria, viruses, and spores. The application of heat and pressure in an autoclave creates an environment that is inhospitable to pathogens, ensuring that surgical instruments, laboratory glassware, and other tools are thoroughly sterilized before use. This feature is critical in maintaining a safe and clean environment for both patients and healthcare providers. While the other options may involve processes that contribute to cleanliness and safety, they do not fulfill the primary function of sterilization in the way that an autoclave does.